Pemanfaatan Bakteri Selulolitik Dan Xilanolitik Yang Potensial Untuk Dekomposisi Jerami Padi

Hasrul Satria Nur ¹, Anja Meryandini ^2,3, Hamim ³

ABSTRACT

Utilization of Potential Cellulolytic and Xilanolytic Bacteria for Decomposition of Rice Straw (Hasrul Satria Nur, Anja Meryandini, and Hamim). There were 3 prospective isolates of cellulolytic bacteria resulted from the total of 31 isolates we found, i.e. C4-4, C5-1, and C11-1. Four combinations of bacteria including C4-4 + Xilanolytic (A), C5-1 + Xilanolytic (B), C11-1 + Xilanolytic (C), 45I-3 + 234P-16 (D), and control (E, without bacteria) were applied as inoculant of rice straw decomposition. In the incubation period the pH-H₂O value of C4-4 + Xilanolytic (A) and C5-1 + Xilanolytic (B) was relatively stable. The C/N ratio of all treatments decreased after 3 weeks of incubation. The C/N ratio value of A, B, C, D and E treatments were 22.48, 23.43, 27.49, 26.82, and 29.53 respectively. Decomposition rate all of combination treatments were faster than the control. The content of macro-micro nutrient of A, B, C, and D treatments increased in the end of measurement, while the control didn’t. The physical characteristic of substrate after incubation was better in A and B treatments that others. The result indicated that the combination of C4-4 + Xilanolytic (A) and C5-1 + Xilanolytic (B) bacteria were the best combination for decomposition of rice straw.

Key words: Cellulolytic, Decomposition, Rice straw, Xilanolytic¹

11 Program Studi Bioteknologi Sekolah Pascasarjana Institut Pertanian Bogor

2 Pusat Penelitian Sumberdaya Hayati dan Bioteknologi IPB Dramaga Bogor

3 Departemen Biologi FMIPA Institut Pertanian Bogor

* Korespondensi. Hasrul Satria Nur, Bag Mikrobiologi Lab Dasar FMIPA Unlam, Jl Jend. A. Yani Km 36 Banjarbaru Kalimantan Selatan 70714, Telp/Fax 0511-4772826. Email: satrianch@yahoo.com

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